Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (135,145)

Singleplex and Multiplex Bead Based Immunoassays (6,198)

ELISA Substrates and Reagents (2,230)

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301 – 315 of 141,015 results

301

Invitrogen™ Human/Mouse TGF beta-1 Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human,Mouse
Conjugate	HRP
Gene Symbol	Tgfb1
Total Assay Time	24 hr 30 min
Sample Volume	Plasma 20 μL, Serum 20 μL, Supernatant 100 μL
Assay Sensitivity	15.6 pg/mL
Storage Requirements	2-8°C
Assay Range	15.6-1000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 45 min
Accession Number	P01137, P04202
Regulatory Status	RUO
Target	TGF beta-1
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Detection Enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,Coating Buffer: 10X PBS ELISA Coating Buffer,Assay Diluent: 5X Concentrated,Detection Enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	21803, 7040
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin
Synonym	Transforming Growth Factor beta 1, TGF-b1

302

Invitrogen™ Human MCP-1 ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	8.7%
Intraassay CV	4.7
Gene Symbol	GDCF-2,HC11,HSMCR30,MCAF,MCP-1,MCP1,SCYA2,SMC-CF
Total Assay Time	2 hrs 10 mins
Sample Volume	Amniotic Fluid 20 μL, Plasma 20 μL, Serum 20 μL, Supernatant 20 μL
Assay Sensitivity	2.3 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	15.6 to 1,000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 20 mins
Accession Number	P13500
Regulatory Status	RUO
Target	CCL2 (MCP-1)
Kit Contents	Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Controls,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	6347
Sample Type	Amniotic Fluid,Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Monocyte chemoattractant protein 1, MCAF, HC11

303

Invitrogen™ Mouse IL-25/IL-17E ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Intraassay CV	6.5%
Total Assay Time	3 hr 30 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	0.9 pg/mL
Assay Range	3.9-250 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Regulatory Status	RUO
Target	IL-25
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	140806
Sample Type	Plasma,Serum,Supernatant
Synonym	Interleukin-17 E, Interleukin-25

304

Invitrogen™ Ferric Antioxidant Status Detection Kit

Assay Ferric Antioxidant Power (FRAP) in Serum, Plasma, Urine, Teas, Fruit Juices, Beer, Cider, Cell Lysates, Herbal and Fruit Extracts.

Pricing & Availability
Specifications

Shipping Condition	Approved for shipment on Wet or Dry Ice
Immunoassay Kit Format	Colorimetric Detection Kit
Content And Storage	96-well plate(s), standard, other assay specific reagents, buffers, and lot-specific technical data sheet. Store at –20°C.
For Use With (Equipment)	Microplate Reader
Product Type	Ferric Antioxidant Status Detection Kit
Gene ID (Entrez)	Non-Gene
Sample Type	Other Biological Fluids,Plasma,Serum
Gene Symbol	Non-Gene
Target Kit Named	Ferric Antioxidant Status
Detection Method	Colorimetric

305

Invitrogen™ Mouse IL-13 Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Gene Symbol	RATTNF,TNF-alphaa,Tnf
Total Assay Time	24 hrs 30 mins
Sample Volume	Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	4 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	4 to 500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P20109
Regulatory Status	RUO
Target	IL-13
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,eBioscience ELISA/ ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	16163
Sample Type	Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-13, IL13

306

Invitrogen™ Multispecies ERK1/ERK2 (Total/Phospho) InstantOne™ ELISA Kit

ELISA

Pricing & Availability

307

Invitrogen™ Mouse IFN gamma Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Gene Symbol	BCGF-1,BCGF1,BSF-1,BSF1,IL-4
Total Assay Time	24 hrs 30 mins
Sample Volume	Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	15 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	15 to 2,000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P01580
Regulatory Status	RUO
Target	IFN gamma
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	15978
Sample Type	Serum,Supernatant
Label or Dye	HRP
Synonym	Interferon-gamma

308

Invitrogen™ Rat TNF alpha ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Rat
Conjugate	HRP
Interassay CV	10%
Intraassay CV	5%
Total Assay Time	3 hr 10 min
Sample Volume	Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	11.0 pg/mL
Storage Requirements	2-8°C
Assay Range	39.1-2500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Accession Number	P16599
Regulatory Status	RUO
Target	TNF alpha
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	24835
Sample Type	Serum,Supernatant
Label or Dye	Biotin
Synonym	Cachectin, Tumor necrosis factor alpha, DIF

309

Invitrogen™ Human Ghrelin ELISA Kit

ELISA

Pricing & Availability
Specifications

For Use With (Equipment)	Colorimetric Microplate Reader
Gene ID (Entrez)	51738
Interassay CV	8.5%
Intraassay CV	6
Assay Sensitivity	11.8 pg/mL
Assay Range	15.6 to 1,000.0 pg/mL
For Use With (Application)	ELISA

310

Invitrogen™ Bovine bFGF ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10
Gene Symbol	FGF2
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	4.1 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	4.10 to 1,000 pg/mL
Test Time	1 hrs 20 mins
Accession Number	Basic fibroblast growth factor; basic fibroblast growth factor bFGF; bFGF; FGF; fgf basic; Fgf2; Fgf-2; FGFB; FGF-b; Fibroblast growth factor; Fibroblast growth factor 2; fibroblast growth factor 2 (basic); HBGF-2; Heparin-binding growth factor 2; H-FGF-b-147; H-FGF-b-154; M-FGF-b; Prostatic growth factor; prostatropin
Regulatory Status	RUO
Target	FGF2
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	281161
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

311

Invitrogen™ Human IL-4 ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	5.6%
Intraassay CV	4.8%
Gene Symbol	Il4
Total Assay Time	3 hr 10 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	1.3 pg/mL
Storage Requirements	2-8°C
Assay Range	7.8-500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Accession Number	P05112
Regulatory Status	RUO
Target	IL-4
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3565
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin
Synonym	B-cell stimulatory factor 1, B cell growth factor 1

312

R&D Systems™ Human Lipocalin-2/NGAL Quantikine ELISA Kit

Human Lipocalin-2/NGAL Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability
Specifications

Specificity	Natural and Recombinant Human Lipocalin-2
Target Species	Human
Conjugate	HRP
Product Type	Solid Phase Sandwich ELISA
Gene ID (Entrez)	3934
Sample Type	Cell Culture Supernatant,Plasma (Heparin),Saliva,Serum,Urine
Sample Volume	Cell Culture Supernates (50uL),Serum (50uL),Heparin Plasma (50uL),Saliva (50uL),Urine (50uL)
Synonym	24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, HNL, LCN2, lipocalin 2, lipocalin 2 (oncogene 24p3), Lipocalin2, Lipocalin-2, migration-stimulating factor inhibitor, MSFI, neutrophil gelatinase-associated lipocalin, NGAL, NGALlipocalin-2, Oncogene 24p3, p25, Siderocalin, Uterocalin
Assay Sensitivity	0.04ng/mL
Storage Requirements	Store the unopened product at 2° to 8°C. Do not use past expiration date.
Assay Range	0.16 - 10ng/mL
For Use With (Application)	ELISA
Cross Reactivity	<50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules.

313

Invitrogen™ Human IL-6 Receptor (Soluble) ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	2.2%
Intraassay CV	1.7
Gene Symbol	CD126,IL-6 R-1,IL-6 RA,IL6QA,IL6RQ,gp80,IL6R
Total Assay Time	2 hrs 10 mins
Sample Volume	Amniotic Fluid 20 μL, Plasma 10 μL, Serum 10 μL, Supernatant 20 μL, Urine 20 μL
Assay Sensitivity	0.01 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	0.08 to 5.0 ng/mL
For Use With (Application)	ELISA
Test Time	1 hrs 20 mins
Accession Number	P08887
Regulatory Status	RUO
Target	IL-6 Receptor (CD126)
Kit Contents	Pre-coated 96 well plates,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3570
Sample Type	Amniotic Fluid,Plasma,Serum,Supernatant,Urine
Label or Dye	HRP
Synonym	CD126, Membrane glycoprotein 80

314

Invitrogen™ Human IL-8 Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	GMCSF
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	2 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	2 to 250 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P10145
Regulatory Status	RUO
Target	IL-8
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,Coating Buffer: 10X PBS ELISA Coating Buffer,Assay Diluent: 5X Concentrated,Detection Enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22 and -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3576
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-8, CXCL8, NAP-I

315

Invitrogen™ Aldosterone Competitive ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Description	Aldosterone Competitive ELISA Kit
Interassay CV	19.5%
Intraassay CV	6.9
Total Assay Time	16 hrs 45 mins
Sample Volume	Fecal Extract 100 μL, Plasma 100 μL, Serum 100 μL, Supernatant 100 μL, Urine 25 μL
Assay Sensitivity	4.97 pg/mL
Storage Requirements	-20°C
Research Discipline	Reproductive Biology
Assay Range	3.906 to 4000 pg/mL
For Use With (Application)	ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
Test Time	1 hrs 20 mins
Regulatory Status	RUO
Target	Aldosterone
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,HRP-Conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Sample Type	Fecal Extract,Plasma,Serum,Supernatant,Urine
Label or Dye	HRP

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R&D Systems™ Human Lipocalin-2/NGAL Quantikine ELISA Kit GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

R&D Systems™ Human Lipocalin-2/NGAL Quantikine ELISA Kit