Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (131,857)

Singleplex and Multiplex Bead Based Immunoassays (5,864)

ELISA Substrates and Reagents (2,338)

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301 – 315 of 137,516 results

301

Invitrogen™ Mouse IgA Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
Assay Sensitivity	0.39 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	0.39 to 25 ng/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Regulatory Status	RUO
Target	Mouse IgA
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	238447
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Immunoglobulin A

302

Invitrogen™ Mouse IgG (Total) Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
Assay Sensitivity	1.56 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	1.56 to 100 ng/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Regulatory Status	RUO
Target	Mouse IgG
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Immunoglobulin G, Immunoglobulin G total, IgG-total

303

Invitrogen™ Human IL-8 Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	2 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	2 to 250 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P10145
Regulatory Status	RUO
Target	IL-8
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3576
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-8, CXCL8, NAP-I

304

Invitrogen™ Human IL-8 Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	GMCSF
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	2 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	2 to 250 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P10145
Regulatory Status	RUO
Target	IL-8
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,Coating Buffer: 10X PBS ELISA Coating Buffer,Assay Diluent: 5X Concentrated,Detection Enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22 and -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3576
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-8, CXCL8, NAP-I

305

Invitrogen™ Mouse PLGF-2 (PGF) ELISA Kit

ELISA

Pricing & Availability
Specifications

Protein Family	Growth Factors & Receptors
Target Species	Mouse
Conjugate	HRP
Intraassay CV	<10
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Assay Sensitivity	1 pg/mL
Research Discipline	Development
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hrs 20 mins
Assay	Solid-phase sandwich ELISA
Target	PGF
Specificity	The sandwich ELISA antibody pair detects Mouse PLGF-2.
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Label or Dye	HRP
Sufficient For	96 Assays
Calibration Range	1 pg/mL to 400 pg/mL
Interassay CV	<12%
Gene Symbol	Pgf
Storage Requirements	2°C to 8°C
Assay Range	1.64 to 400 pg/mL
Accession Number	P49764
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	18654
Sample Type	Plasma,Serum,Supernatant
Synonym	AI854365, PIGF, Plgf

306

Invitrogen™ Human Osteoprotegerin (TNFRSF11B) Instant ELISA™ Kit

ELISA

Pricing & Availability
Specifications

For Use With (Equipment)	Colorimetric Microplate Reader
Gene ID (Entrez)	4982
Interassay CV	8%
Intraassay CV	7
Assay Sensitivity	2.5 pg/mL
Assay Range	15.6 to 1,000 pg/mL
For Use With (Application)	ELISA

307

Invitrogen™ Human IL-23 Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	AI553453,CCP-1/C11,CCP1,Ctla-1,Ctla1,GZB
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	15 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	15 to 2000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	Q9NPF7
Regulatory Status	RUO
Target	IL-23
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	51561
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-23, IL23, p40, p19

308

Invitrogen™ Mouse IL-17A (homodimer) Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Gene Symbol	AV080368,Mcp-1
Total Assay Time	24 hrs 30 mins
Sample Volume	Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	4 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	4 to 500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	Q62386
Regulatory Status	RUO
Target	IL-17 A
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,ELISA/ELISPOT Coating Buffer Powder: This Uncoated ELISA Kitmay contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	16171
Sample Type	Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-17 A, IL-17 AA

309

Invitrogen™ Human 4-1BB Ligand ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10
Gene Symbol	TNFSF9
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	0.2 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	0.2 to 50 ng/mL
Test Time	1 hrs 20 mins
Accession Number	4 1 BB Ligand; 4 1 BBL; 41 BB Ligand; 4-1 BB ligand; 41 BBL; 4-1 BBL; 4-1 BB-L; AI848817; CD 137 Ligand; CD 137 L; CD137 Ligand; CD137L; Cd157l; CDw137L; costimulatory molecule; homolog of mouse 4-1 BB-L; Ly63l; receptor 4-1 BB ligand; TNFRSF9; Tnfsf9; TNLG5A; tumor necrosis factor (ligand) superfamily member 9; tumor necrosis factor (ligand) superfamily, member 9; tumor necrosis factor ligand 5 A; tumor necrosis factor ligand superfamily member 9; tumor necrosis factor superfamily member 9
Regulatory Status	RUO
Target	4-1 BBL (CD137L)
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	8744
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

310

Invitrogen™ pNPP Solution

Suitable for use in immunoassays

Pricing & Availability

311

Invitrogen™ TMB Chromogen Solution (for ELISA)

May be used as a substrate for horseradish peroxidase (HRP) in ELISAs

Pricing & Availability

312

Invitrogen™ Human TNF alpha Instant ELISA™ Kit

ELISA

Pricing & Availability

313

Invitrogen™ Human IL-1 beta ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	8.6%
Intraassay CV	5.1
Gene Symbol	IL-1,IL1-BETA,IL1F2
Total Assay Time	3 hrs 10 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 50 μL
Assay Sensitivity	0.3 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	3.9 to 250 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 20 mins
Accession Number	P01584
Regulatory Status	RUO
Target	IL-1 beta
Kit Contents	Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Controls,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3553
Sample Type	Plasma,Serum,Supernatant,Urine
Label or Dye	HRP
Synonym	Catabolin, Interleukin-1 beta, IL1F2

314

Invitrogen™ Mouse IP-10 (CXCL10) ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Interassay CV	7.2%
Intraassay CV	8.4
Gene Symbol	C7,CRG-2,INP10,IP-10,IP10,Ifi10,Scyb10,gIP-10,mob-1
Total Assay Time	3 hrs 10 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	6.5 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	7.8 to 500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 20 mins
Accession Number	P17515
Regulatory Status	RUO
Target	CXCL10 (IP-10)
Kit Contents	Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	15945
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Interferon-gamma-inducible protein 10, CXCL10

315

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R&D Systems™ Human IL-1 beta/IL-1F2 Quantikine ELISA Kit

Human IL-1 beta/IL-1F2 Quantikine ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Pricing & Availability
Specifications

Specificity	Natural and Recombinant Human IL-1 beta
Target Species	Human
Conjugate	HRP
Product Type	Solid Phase Sandwich ELISA
Gene ID (Entrez)	3553
Sample Type	Cell Culture Supernatant,Plasma (EDTA, Citrate, Heparin),Serum
Sample Volume	Cell Culture Supernates (200uL),Serum (200uL),EDTA Plasma (200uL),Heparin Plasma (200uL),Citrate Plasma (200uL)
Synonym	catabolin, IL-1, IL1 beta, IL-1 beta, IL-1 b, IL1B, IL1-BETA, IL-1F2, IL1F2IL-1 beta, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta
Assay Sensitivity	1pg/mL
Storage Requirements	Store the unopened product at 2° to 8°C. Do not use past expiration date.
Assay Range	3.90 - 250pg/mL
For Use With (Application)	ELISA
Cross Reactivity	<50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules

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R&D Systems™ Human IL-1 beta/IL-1F2 Quantikine ELISA Kit GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

R&D Systems™ Human IL-1 beta/IL-1F2 Quantikine ELISA Kit